|
Translation System
|
Nucleic Acid Template
|
Promoter |
RBS (1)
|
Features
|
Protein
Yield (per 50 µl rxn) |
|
RNA
|
DNA circular
|
DNA linear (plasmid
or PCR product)
|
| PROTEINscript™ II |
No
|
Yes
|
Yes
|
T7
|
Not required (2)
|
- Linked transcription and translation.
- No subcloning prior to RNA transcript synthesis or purification
of mRNA required
- Uses vectors or PCR products directly.
|
50-200 ng
|
| Retic
Lysate IVT™ (treated) |
Yes
|
No
|
No
|
Not required
|
Not required (2)
|
- Treated so that retic mRNAs are destroyed
- Only exogenous mRNAs, both capped and uncapped, are translated
- Ideal for translation of proteins >20 kDa
|
50-250 ng
|
(1)
Recommended ribosome binding site (RBS).
(2) Kozak sequence may
improve fidelity of translation.
|